Anatid herpesvirus 1 CH virulent strain induces syncytium and apoptosis in duck embryo fibroblast cultures

Anatid herpesvirus 1 (AHV-1) CH virulent strain was first isolated from an infected duck and it was found that this virus strain could induce cytopathic effect (CPE) in duck embryo fibroblast (DEF). Following AHV-1 infection, DEF showed morphological changes such as cell rounding, improved refractivity and detachment from the culture surface. However, its pathological characteristics were not adequately known. Related studies were performed and the results showed that syncytium formation could be observed as the other type of CPE in AHV-1 infection. Hematoxylin-eosin staining and 4’, 6-diamidino-2-phenylindole (DAPI) staining of infected DEF were each used to visualize the shape and distribution of chromatin within nuclei and nuclear fragmentation was observed. Chromatin condensation and margination, as well as formation of apoptotic bodies were observed by transmission electron microscopy (TEM). DNA ladder formation was detected in AHV-1 infected cells and apoptosis of the infected DEF was also detected by flow cytometry analysis of Annexin V-FITC/PI staining method. Therefore, it was suggested that AHV-1 virulent strain can induce syncytium and apoptosis in DEF. Syncytium formation and apoptosis observed in this study may contribute to the elucidation of AHV-1 pathogenesis.     

水解蜂蛹蛋白对大鼠免疫功能的影响

为了研究水解蜂蛹蛋白对大鼠免疫功能的影响及性别之间的差异,选用50只3周龄SD大鼠随机分成2组,分别灌胃蜂蛹蛋白粉和经消化酶水解的蜂蛹蛋白4周.结果表明,饲喂水解蜂蛹蛋白雌鼠胸腺指数比对照组提高30.00%(p＜0.05),雄鼠脾脏指数比对照组提高8.33%(p＜0.05),而雌鼠脾脏指数和雄鼠胸腺指数与对照组无显著差异.饲喂水解蜂蛹蛋白组雌鼠和雄鼠血清IgG的含量分别比对照组增加21.82%(p＜0.05)和41.34%(P＜0.05);雌鼠血清IgA含量比对照组增加52.78%(0＜0.01).结果提示,水解蜂蛹蛋白有增强大鼠的免疫功能的作用,且存在一定的性别差异.     

开放条件下中国棉花产业安全研究

我国是世界棉花生产和消费大国.开放条件下,我国棉花的机械化生产水平不断提高,但国内棉花供应仍处于供不应求的状态,棉花贸易长期处于净进口局面.同时,棉花产业发展也深受贸易摩擦和某些国家政治打压的影响.基于我国棉花生产和贸易数据,运用进口市场集中度、进口依存度、出口依存度、自给率、国际竞争力等指标对我国棉花产业安全程度进行...     

动物流行病学正态样本异常值的判断和处理

讨论动物流行病学调查分析数据正态样本的判断和处理。描述格拉布斯检验法和偏度-峰度检验法在处理正态样本中的步骤。以仔猪市场价格为例,阐述格拉布斯检验法的运用方法。说明样本异常值的处理技术。     

猪繁殖与呼吸综合征病毒双基因多组合DNA疫苗的构建

本实验根据PRRSV结构蛋白的特性和IFNγ及IL-2这两种细胞因子的特性,构建表达PRRSVGP4-GP5、GP5-M、GP5-N、GP5-IFNγ、GP5-IL-2、N-IFNγ、N-IL-2的重组质粒。这些重组质粒在真核细胞中得到了表达。用这些真核表达质粒可以免疫小猪,通过检测其对猪体免疫应答的诱导能力和接种后动物对PRRSV的抵抗力来对基因疫苗的效能进行研究,以期筛选到有效的基因疫苗并为今后进一步的研究提供思路和理论基础。     

两株不同地区副猪嗜血杆菌的分离及分子生物学鉴定

本试验从浙江和河南部分地区的送检的疑似患多发浆膜炎与关节炎猪的病料中分离到2株疑似副猪嗜血杆菌,进行了细菌形态观察、培养特性和生化特性鉴定,根据已报道的副猪嗜血杆菌16SrRNA序列特异性引物进行PCR扩增,得到预期的822bp的基因片断,最终证实为副猪嗜血杆菌。     

百年基业何许来,唯有智慧是源头

花开只是春的朦胧,春的起始,花落才是春的永恒,春的赠予。市场行情如同花开花落,好与坏、高与低、晴与阴、旦与夕相随相伴。但我相信在这瞬息万变的市场行情中总有一部分优秀经销商能笑傲江湖,福满人间,春满心甜。     

疫苗免疫需注意的几个方面

一搞好防暑降温 1舍内降温 开放式鸡舍应安装吊扇,加速空气流动降温;密闭式鸡舍可采用纵向通风法降温,即在鸡舍前门设置进风口,后门设置排风口,依舍内空间大小设置一定数量的排气扇,增强对流散热效果。注意通风时将两侧窗户关闭,使舍内任何部位均能感觉到凉风拂面。还可在进风处设置水帘．使外界热空气经过冷却后再进入鸡舍。     

节约成本的禽舍建造技术

未来家禽业的发展将面临许多问题。但有一件事是肯定的,那就是多数专家认为,随着丙烷燃气与电力使用成本的不断上升,禽舍的设计与建造将不得不比过去更加关注运营成本,特别是加热与散热成本。